Data documento
25/12/2024

Título
A importância da hidratação e da água potável para sua saúde

Autor(es)
Mariana Pioltine Murari

Palavras chave
bem-estar - desidratação

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
04/10/2024

Título
A relação entre hormônios e saúde mental

Autor(es)
Ana Carolina Teixeira

Palavras chave
bem-estar - cortisol

Descrição
Orientadora: Prof.ª Larissa Donadel Barreto Sargentini

Data documento
06/05/2024

Título
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients

Autor(es)
Teixeira S.A. - Burim R.V. - Viapiano M.S. - Bidinotto L.T. - Nagashi Marie S.K. - Fleury Malheiros S.M. - Oba-Shinjo S.M. - Andrade A.F. - Carlotti C.G.

Palavras chave
ITGA2 - brain microenvironment - extracellular matrix - invasion - low grade glioma - single nucleotide polymorphism - tumor progression

Resumo
Integrins are heterodimeric transmembrane glycoproteins resulting from the non-covalent association of an α and β chain. The major integrin receptor for collagen/laminin, α2β1 is expressed on a wide variety of cell types and plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Integrin-triggered signaling pathways promote the invasion and survival of glioma cells by modifying the brain microenvironment. In this study, we investigated the association of a specific genetic polymorphism of integrin α2β1 with the incidence of diffusely infiltrating astrocytoma and the progression of these tumors. Single-nucleotide polymorphism in intron 7 of the integrin ITGA2 gene was examined in 158 patients and 162 controls using polymerase chain reaction and restriction enzyme analysis. The ITGA2 genotype +/+ (with a BglII restriction site in both alleles) exhibited higher frequency in grade II astrocytoma compared to control (P = 0.02) whereas the genotype -/- (lacking the BglII site) correlated with the poorest survival rate (P = 0.04). In addition, in silico analyses of ITGA2 expression from low-grade gliomas (LGG, n = 515) and glioblastomas (GBM, n = 159) indicated that the higher expression of ITGA2 in LGG was associated with poor overall survival (P < 0.0001). However, the distribution of integrin ITGA2 BglII genotypes (+/+, +/-, -/-) was not significantly different between astrocytoma subgroups III and IV (P = 0.65, 0.24 and 0.33; 0.29, 0.48, 0.25, respectively) compared to control. These results suggest a narrow association between the presence of this SNP and indicate that further studies with larger samples are warranted to analyze the relation between tumor grade and overall survival, highlighting the importance of determining these polymorphisms for prognosis of astrocytomas.

Citação
Teixeira SA, Burim RV, Viapiano MS, Bidinotto LT, Nagashi Marie SK, Fleury Malheiros SM, Oba-Shinjo SM, Andrade AF, Carlotti CG. Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients. Front Oncol. 2022 Jul 22;12:914156. doi: 10.3389/fonc.2022.914156. PMID: 35936750; PMCID: PMC9353741.

Data documento
29/10/2023

Título
Artrite reumatoide: diagnóstico e estratégias de tratamento

Autor(es)
Ellen Maciel Pires

Palavras chave
doença crônica - articulações

Descrição
Orientador: Prof. Robson Aparecido dos Santos Boni

Data documento
09/06/2024

Título
Artrite reumatóide: sintomas e tratamentos

Autor(es)
Larissa Bertaggia Giacomelli

Palavras chave
AR - doença inflamatória crônica

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
05/01/2025

Título
Atividade física e prevenção de doenças cardiovasculares

Autor(es)
Milena Antunes Vieira

Palavras chave
atividade física - doenças cardiovasculares

Descrição
Orientador: Prof. Celina Antonio Prata

Data documento
01/12/2020

Título
Atuação do enfermeiro em unidade de terapia intensiva no cuidado de pacientes adultos submetidos à cirurgia cardíaca

Autor(es)
Flávia Maia Bovarotti - Ludimila Angélica Feltrin

Palavras chave
UTI - Cirurgia cardíaca - Assistência de enfermagem

Resumo
JUSTIFICATIVA: A cirurgia cardíaca requer ações da equipe de saúde que visam garantir atendimento de qualidade ao paciente, resultando em rápida recuperação e desospitalização precoce. A complexidade do cliente submetido à cirurgia cardíaca e sua hemodinâmica instabilidade são fatores importantes para a observação contínua de enfermeiros e sua equipe. Portanto, no campo de atuação do enfermeiro, as doenças cardíacas correspondem a importante demanda de cuidados, justificando um olhar sistematizado para esse grupo de agravos, na perspectiva da integralidade da atenção. OBJETIVO: Identificar a importância do enfermeiro frente ao atendimento de pacientes adultos que foram submetidos a cirurgias de âmbito cardíaco, onde se encontram em recuperação na Unidade de Terapia Intensiva, relacionando com suas condutas e conhecimentos. MATERIAIS E MÉTODOS: Revisão integrativa realizada por meio do levantamento de dados nas bases virtuais MEDLINE via PubMed, Cochrane Library e Biblioteca Virtual da Saúde (BVS) LILACs, com limitação temporal de 10 anos. RESULTADOS: Foram encontrados 588 artigos a partir da busca por descritores, sendo 98 da PubMed, 352 da Biblioteca Virtual de Saúde e 138 da Cochrane Library. Destes, foram selecionados 28 artigos para revisão, e 09 referenciados. CONCLUSÃO: Os cuidados envolvidos em Unidade de Terapia Intensiva no pós operatório de cirurgia cardíaca demonstram necessidade de organização no processo de trabalho da equipe de enfermagem, bem como competências dos profissionais para atuarem nesse cenário, com intuito de promover assistência de qualidade, pautada nas orientações necessárias.

Descrição
Trabalho de Conclusão de Curso (Pós Graduação em Enfermagem em Emergência e Terapia Intensiva)

Citação
BOVAROTTI, Flávia Maia; FELTRIN, Ludimila Angelica. Atuação do enfermeiro em Unidade de Terapia Intensiva no cuidado de pacientes adultos submetidos à cirurgia cardíaca. 2020. 18 f. Trabalho de Conclusão de Curso (Pós Graduação em Enfermagem em Emergência e Terapia Intensiva) - Faculdade de Ciências da Saúde de Barretos Dr. Paulo Prata, 2020.

Data documento
06/08/2025

Título
AVC: como identificar, agir e reduzir os riscos

Autor(es)
Karoline Cristina da Silva Lucino

Palavras chave
AVC - Acidente vascular cerebral

Descrição
Orientador: Prof. Otávio Costa Vicenzi

Data documento
29/05/2024

Título
AVC: Uma corrida contra tempo

Autor(es)
Isadora Souza Caobianco

Palavras chave
acidente vascular cerebral

Descrição
Orientador: Prof. Robson Aparecido dos Santos Boni

Data documento
19/06/2025

Título
Bronzeamento artificial: entenda os riscos para a sua pele

Autor(es)
Maria Eduarda Pereira

Palavras chave
bronzeamento artificial

Descrição
Orientador: Prof. Bruno Augusto Alvares

Data documento
13/06/2024

Título
C10H14N2: Desdobramentos dos "E-Cig" no Psicológico Juvenil

Autor(es)
Maria Gabriela Aquino Mendes

Palavras chave
cigarro eletrônico

Descrição
Orientadora: Prof.ª Barbara Sgavioli Massucato

Data documento
30/03/2023

Título
Câncer de Pulmão: a chave ainda é a prevenção e o diagnóstico precoce

Autor(es)
Alessandro Pascon Filho

Palavras chave
cigarro - fumantes

Descrição
Orientadora: Prof.ª Leticia Ferro Leal

Data documento
17/01/2024

Título
Câncer renal: como prevenir?

Autor(es)
Marina Gomes da Silva

Palavras chave
rins - carcinome de células renais

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
06/05/2024

Título
Clinical Course, Genetic, and Immunohistochemical Characterization of Solid Pseudopapillary Tumor of the Pancreas (Frantz Tumors) in a Brazilian Cohort

Autor(es)
Tostes F.T. - de Carvalho P.F.D.C. - Araújo R.L.C. - Ribeiro R.C. - Apodaca-Torrez F.R. - Lobo E.J. - Gomes D.B.D. - Callegaro-Filho D. - Schvartsman G. - Moura F. - Schraibman V. - Goldenberg A. - de Lima F.T. - Segatelli V. - Uson Junior P.L.S.

Palavras chave
Frantz tumor - Li-Fraumeni syndrome - pancreatic tumor - solid pseudopapillary pancreatic neoplasm

Resumo
Frantz tumors or solid pseudopapillary pancreatic neoplasm (SPN) are rare exocrine neoplasms that carry a favorable prognosis; they represent up to 3% of all tumors located in the region of the pancreas and have specific age and gender predispositions. In recent years, the rising curve of diagnosis is entitled to the evolution and access of diagnostic imaging. In this paper, we have retrospectively reviewed and described the clinical course of 40 patients with SPN from three institutions in Brazil, who had their diagnosis between 2005 and 2020, and analyzed the clinicopathological, genetic, and surgical aspects of these individuals. In accordance with the literature, most patients were women, 60% with unspecified symptoms at diagnosis, with tumors mainly located in the body and tail of the pancreas, of whom 70% underwent a distal pancreatectomy with sparing splenectomy as a standard procedure, and none of the cases have experienced recurrence to date. Surgery still remains the mainstay of treatment given the low metastatic potential, but more conservative approaches as observed in this cohort are evolving to become the standard of care. Herein, we present an in-depth analysis of cases focusing on the latest literature and report some of the smallest tumor cases in the literature. To our knowledge, this is the first report evaluating germline genetic testing and presenting a case of detected Li-Fraumeni syndrome.

Citação
Tostes FT, de Carvalho PFDC, Araújo RLC, Ribeiro RC, Apodaca-Torrez FR, Lobo EJ, Gomes DBD, Callegaro-Filho D, Schvartsman G, Moura F, Schraibman V, Goldenberg A, de Lima FT, Segatelli V, Uson Junior PLS. Clinical Course, Genetic, and Immunohistochemical Characterization of Solid Pseudopapillary Tumor of the Pancreas (Frantz Tumors) in a Brazilian Cohort. Genes (Basel). 2022 Oct 6;13(10):1809. doi: 10.3390/genes13101809. PMID: 36292694; PMCID: PMC9601385.

Data documento
08/10/2023

Título
Como prevenir doenças e ter uma vida mais saudável?

Autor(es)
Ana Beatriz Franco Santos

Palavras chave
alimentação - sedentarismo - hábitos saudáveis

Descrição
Orientadora: Prof.ª Patricia Modiano

Data documento
06/11/2024

Título
Como reconhecer um infarto?

Autor(es)
Débora Mazzochi Banck

Palavras chave
miocárdio - músculo cardíaco

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
06/05/2024

Título
Comprehensive Molecular Landscape of Cetuximab Resistance in Head and Neck Cancer Cell Lines

Autor(es)
Gomes I.N.F. - da Silva-Oliveira R.J. - da Silva L.S. - Martinho O. - Evangelista A.F. - van Helvoort Lengert A. - Leal L.F. - Silva V.A.O. - Dos Santos S.P. - Nascimento F.C. - Lopes Carvalho A. - Reis R.M.

Palavras chave
EGFR - biomarkers - cetuximab - drug resistance - head and neck tumors - in vitro - pre-clinical

Resumo
Cetuximab is the sole anti-EGFR monoclonal antibody that is FDA approved to treat head and neck squamous cell carcinoma (HNSCC). However, no predictive biomarkers of cetuximab response are known for HNSCC. Herein, we address the molecular mechanisms underlying cetuximab resistance in an in vitro model. We established a cetuximab resistant model (FaDu), using increased cetuximab concentrations for more than eight months. The resistance and parental cells were evaluated for cell viability and functional assays. Protein expression was analyzed by Western blot and human cell surface panel by lyoplate. The mutational profile and copy number alterations (CNA) were analyzed using whole-exome sequencing (WES) and the NanoString platform. FaDu resistant clones exhibited at least two-fold higher IC50 compared to the parental cell line. WES showed relevant mutations in several cancer-related genes, and the comparative mRNA expression analysis showed 36 differentially expressed genes associated with EGFR tyrosine kinase inhibitors resistance, RAS, MAPK, and mTOR signaling. Importantly, we observed that overexpression of KRAS, RhoA, and CD44 was associated with cetuximab resistance. Protein analysis revealed EGFR phosphorylation inhibition and mTOR increase in resistant cells. Moreover, the resistant cell line demonstrated an aggressive phenotype with a significant increase in adhesion, the number of colonies, and migration rates. Overall, we identified several molecular alterations in the cetuximab resistant cell line that may constitute novel biomarkers of cetuximab response such as mTOR and RhoA overexpression. These findings indicate new strategies to overcome anti-EGFR resistance in HNSCC.

Citação
Gomes INF, da Silva-Oliveira RJ, da Silva LS, Martinho O, Evangelista AF, van Helvoort Lengert A, Leal LF, Silva VAO, Dos Santos SP, Nascimento FC, Lopes Carvalho A, Reis RM. Comprehensive Molecular Landscape of Cetuximab Resistance in Head and Neck Cancer Cell Lines. Cells. 2022 Jan 4;11(1):154. doi: 10.3390/cells11010154. PMID: 35011716; PMCID: PMC8750399.

Data documento
07/05/2024

Título
COVID-19 was not associated or trigger disease activity in spondylarthritis patients: ReumaCoV-Brasil cross-sectional data

Autor(es)
Marques CDL, Ribeiro SLE, Albuquerque CP, de Sousa Studart SA, Ranzolin A, de Andrade NPB, Dantas AT, Mota GD, Resende GG, Marinho AO, Angelieri D, Andrade D, Ribeiro FM, Omura F, Silva NA, Rocha Junior L, Brito DE, Fernandino DC, Yazbek MA, Souza MPG, Ximenes AC, Martins ASS, Castro GRW, Oliveira LC, Freitas ABSB, Kakehasi AM, Gomides APM, Reis Neto ET, Pileggi GS, Ferreira GA, Mota LMH, Xavier RM, de Medeiros Pinheiro M.

Palavras chave
COVID-19 - Hospitalization - Psoriatic arthritis - SARS-CoV-2 - Spondyloarthritis

Resumo
Objectives: To evaluate the disease activity before and after COVID-19 and risk factors associated with outcomes, including hospitalization, intensive care unit (ICU) admission, mechanical ventilation (MV) and death in patients with spondylarthritis (SpA). Methods: ReumaCoV Brazil is a multicenter prospective cohort of immune-mediated rheumatic diseases (IMRD) patients with COVID-19 (case group), compared to a control group of IMRD patients without COVID-19. SpA patients enrolled were grouped as axial SpA (axSpA), psoriatic arthritis (PsA) and enteropathic arthritis, according to usual classification criteria. Results: 353 SpA patients were included, of whom 229 (64.9%) were axSpA, 118 (33.4%) PsA and 6 enteropathic arthritis (1.7%). No significant difference was observed in disease activity before the study inclusion comparing cases and controls, as well no worsening of disease activity after COVID-19. The risk factors associated with hospitalization were age over 60 years (OR = 3.71; 95% CI 1.62-8.47, p = 0.001); one or more comorbidities (OR = 2.28; 95% CI 1.02-5.08, p = 0.001) and leflunomide treatment (OR = 4.46; 95% CI 1.33-24.9, p = 0.008). Not having comorbidities (OR = 0.11; 95% CI 0.02-0.50, p = 0.001) played a protective role for hospitalization. In multivariate analysis, leflunomide treatment (OR = 8.69; CI = 95% 1.41-53.64; p = 0.023) was associated with hospitalization; teleconsultation (OR = 0.14; CI = 95% 0.03-0.71; p = 0.01) and no comorbidities (OR = 0.14; CI = 95% 0.02-0.76; p = 0.02) remained at final model as protective factor. Conclusions: Our results showed no association between pre-COVID disease activity or that SARS-CoV-2 infection could trigger disease activity in patients with SpA. Teleconsultation and no comorbidities were associated with a lower hospitalization risk. Leflunomide remained significantly associated with higher risk of hospitalization after multiple adjustments.

Citação
Marques CDL, Ribeiro SLE, Albuquerque CP, de Sousa Studart SA, Ranzolin A, de Andrade NPB, Dantas AT, Mota GD, Resende GG, Marinho AO, Angelieri D, Andrade D, Ribeiro FM, Omura F, Silva NA, Rocha Junior L, Brito DE, Fernandino DC, Yazbek MA, Souza MPG, Ximenes AC, Martins ASS, Castro GRW, Oliveira LC, Freitas ABSB, Kakehasi AM, Gomides APM, Reis Neto ET, Pileggi GS, Ferreira GA, Mota LMH, Xavier RM, de Medeiros Pinheiro M; ReumaCoV-Brasil Registry. COVID-19 was not associated or trigger disease activity in spondylarthritis patients: ReumaCoV-Brasil cross-sectional data. Adv Rheumatol. 2022 Nov 22;62(1):45. doi: 10.1186/s42358-022-00268-x. PMID: 36419163; PMCID: PMC9685130.

Data documento
07/11/2024

Título
Cuidados Paliativos: Um cuidado para a vida

Autor(es)
Carolina Gerbasi Ricci

Palavras chave
equipe multidisciplinar - humanização

Descrição
Orientador: Prof. Rodrigo Chaves Ribeiro

Data documento
23/06/2023

Título
Entorse e luxação: quando buscar avaliação médica?

Autor(es)
Lucas Augusto Delgado Boteon

Palavras chave
articulação - lesão

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
15/01/2025

Título
Entre algoritmos e estetoscópios: impacto da IA na prática médica

Autor(es)
Victoria Louyse Vaz Constantino

Palavras chave
IA - Inteligência Artificial - prática médica

Descrição
Orientador: Prof. Carlos Sitta Sabaini

Data documento
22/11/2024

Título
Enxaqueca, anticoncepção e riscos de AVC

Autor(es)
Isadora Pavoni Hipólito

Palavras chave
acidente vascular cerebral - neurologia - trombose

Descrição
Orientador: Prof. Otávio Costa Vicenzi

Data documento
02/05/2024

Título
Establishment and molecular characterization of HCB‑541, a novel and aggressive human cutaneous squamous cell carcinoma cell line

Autor(es)
LAUS, Ana Carolina - GOMES, Izabela Natalia Faria - SILVA, Aline Larissa Virginio da - SILVA, Luciane Sussuchi da - MILAN, Mirella Baroni - TEIXEIRA, Silvia Aparecida - MARTINS, Ana Carolina Baptista Moreno - PEREIRA, Letícia do Nascimento Braga - CARVALHO, Carlos Eduardo Barbosa de - CROVADOR, Camila Souza - PAULA, Flávia Escremin de - NASCIMENTO, Flávia Caroline - FREITAS, Helder Teixeira de - VAZQUEZ, Vinicius de Lima - REIAS, Rui Manuel - OLIVEIRA, Renato José da Silva.

Palavras chave
Cutaneous squamous cell carcinoma - Molecular profile - Cell line establishment - In vivo model

Resumo
Cutaneous squamous cell carcinoma (cSCC) is a common type of skin cancer that can result in significant morbidity, although it is usually well-managed and rarely metastasizes. However, the lack of commercially available cSCC cell lines hinders our understanding of this disease. This study aims to establish and characterize a new metastatic cSCC cell line derived from a Brazilian patient. A tumor biopsy was taken from a metastatic cSCC patient, immortalized, and named HCB-541 after several passages. The cytokeratin expression profile, karyotypic alterations, mutational analysis, mRNA and protein differential expression, tumorigenic capacity in xenograft models, and drug sensitivity were analyzed. The HCB-541 cell line showed a doubling time between 20 and 30 h and high tumorigenic capacity in the xenograft mouse model. The HCB-541 cell line showed hypodiploid and hypotetraploidy populations. We found pathogenic mutations in TP53 p.(Arg248Leu), HRAS (Gln61His) and TERT promoter (C228T) and high-level microsatellite instability (MSI-H) in both tumor and cell line. We observed 37 cancer-related genes differentially expressed when compared with HACAT control cells. The HCB-541 cells exhibited high phosphorylated levels of EGFR, AXL, Tie, FGFR, and ROR2, and high sensitivity to cisplatin, carboplatin, and EGFR inhibitors. Our study successfully established HCB-541, a new cSCC cell line that could be useful as a valuable biological model for understanding the biology and therapy of metastatic skin cancer.

Citação
Laus AC, Gomes INF, da Silva ALV, da Silva LS, Milan MB, AparecidaTeixeira S, Martin ACBM, do Nascimento Braga Pereira L, de Carvalho CEB, Crovador CS, de Paula FE, Nascimento FC, de Freitas HT, de Lima Vazquez V, Reis RM, da Silva-Oliveira RJ. Establishment and molecular characterization of HCB-541, a novel and aggressive human cutaneous squamous cell carcinoma cell line. Hum Cell. 2024 Apr 3. doi: 10.1007/s13577-024-01054-1. Epub ahead of print. PMID: 38565739.

Data documento
19/03/2025

Título
Establishment and molecular characterization of HCB-541, a novel and aggressive human cutaneous squamous cell carcinoma cell line.

Autor(es)
Laus AC - Gomes INF - da Silva ALV - da Silva LS - Milan MB - AparecidaTeixeira S - Martin ACBM - do Nascimento Braga Pereira L - de Carvalho CEB - Crovador CS - de Paula FE - Nascimento FC - de Freitas HT - de Lima Vazquez V - Reis RM - da Silva-Oliveira RJ

Palavras chave
Cell line establishment; Cutaneous squamous cell carcinoma; In vivo model; Molecular profile.

Resumo
Cutaneous squamous cell carcinoma (cSCC) is a common type of skin cancer that can result in significant morbidity, although it is usually well-managed and rarely metastasizes. However, the lack of commercially available cSCC cell lines hinders our understanding of this disease. This study aims to establish and characterize a new metastatic cSCC cell line derived from a Brazilian patient. A tumor biopsy was taken from a metastatic cSCC patient, immortalized, and named HCB-541 after several passages. The cytokeratin expression profile, karyotypic alterations, mutational analysis, mRNA and protein differential expression, tumorigenic capacity in xenograft models, and drug sensitivity were analyzed. The HCB-541 cell line showed a doubling time between 20 and 30 h and high tumorigenic capacity in the xenograft mouse model. The HCB-541 cell line showed hypodiploid and hypotetraploidy populations. We found pathogenic mutations in TP53 p.(Arg248Leu), HRAS (Gln61His) and TERT promoter (C228T) and high-level microsatellite instability (MSI-H) in both tumor and cell line. We observed 37 cancer-related genes differentially expressed when compared with HACAT control cells. The HCB-541 cells exhibited high phosphorylated levels of EGFR, AXL, Tie, FGFR, and ROR2, and high sensitivity to cisplatin, carboplatin, and EGFR inhibitors. Our study successfully established HCB-541, a new cSCC cell line that could be useful as a valuable biological model for understanding the biology and therapy of metastatic skin cancer.

Citação
Laus AC, Gomes INF, da Silva ALV, da Silva LS, Milan MB, AparecidaTeixeira S, Martin ACBM, do Nascimento Braga Pereira L, de Carvalho CEB, Crovador CS, de Paula FE, Nascimento FC, de Freitas HT, de Lima Vazquez V, Reis RM, da Silva-Oliveira RJ. Hum Cell. 2024 Jul;37(4):1170-1183. doi: 10.1007/s13577-024-01054-1. Epub 2024 Apr 3. PMID: 38565739

Data documento
09/05/2025

Título
Exposição precoce ao açucar

Autor(es)
Beatriz Castilho Milani

Palavras chave
açucar

Descrição
Orientadora: Prof.ª Thais Kataoka Homma

Data documento
21/06/2025

Título
Fatores de risco por trás do câncer de fígado

Autor(es)
Maria Angélica Tonato de Oliveira

Palavras chave
câncer de fígado - hepatocarcinoma - HCC

Descrição
Orientador: Prof. João Luiz Brisotti

Data documento
03/07/2023

Título
Febre maculosa ou "Doença do Carrapato"

Autor(es)
Guilherme Braatz Marques

Palavras chave
carrapato-estrela

Descrição
Orientadora: Prof.ª Luciana Souza Jorge

Data documento
30/07/2024

Título
Felicidade e bem-estar: quando encontrar um desequilíbrio?

Autor(es)
Luana Castro Gumieri

Palavras chave
bem-estar - saúde mental - saúde física

Descrição
Orientadora: Prof.ª Barbara Sgavioli Massucato

Data documento
19/03/2025

Título
Immune-checkpoint gene expression and BCG response in non-muscle invasive bladder cancer

Autor(es)
Zucca LER, Laus AC - Sorroche BP - Paro E Sussuchi L - Marques RF - Teixeira GR - Berardinelli GN - Arantes LMRB - Reis RM - Cárcano FM.

Palavras chave
Urinary bladder neoplasms1 - BCG vaccine 2 - Gene expression profiling3 - Immunity active4

Resumo
Methods One-hundred-six patients diagnosed with non-muscle invasive bladder cancer and treated with intravesical BCG were included and divided into two groups, BCG-responsive (n = 47) and -unresponsive (n = 59). Immunohistochemistry was used to evaluate PD-L1 expression and MSI was assessed by a commercial multiplex PCR kit. The mRNA expression profile of 15 immune checkpoints was performed using the nCounter technology. For in silico validation, two distinct cohorts sourced from the Gene Expression Omnibus (GEO) database were used. Results Among the 106 patients, only one (<1 %) exhibited MSI instability. PD-L1 expression was present in 9.4 % of cases, and no association was found with BCG-responsive status. We found low gene expression of canonic actionable immune checkpoints PDCD1 (PD-1), CD274 (PD-L1), and CTLA4, while high expression was observed for CD276 (B7-H3), CD47, TNFRSF14, IDO1 and PVR (CD155) genes. High IDO1 expression levels was associated with worst overall survival. The PDCD1, CTLA4 and TNFRSF14 expression levels were associated with BCG responsiveness, whereas TIGIT and CD276 were associated with unresponsiveness. Finally, CD276 was validated in silico cohorts. Conclusion In NMIBC, MSI is rare and PD-L1 expression is present in a small subset of cases. Expression levels of PDCD1, CTLA4, TNFRSF14, TIGIT and CD276 could constitute predictive biomarkers of BCG responsiveness.

Citação
Zucca LER, Laus AC, Sorroche BP, Paro E, Sussuchi L, Marques RF, Teixeira GR, Berardinelli GN, Arantes LMRB, Reis RM, Cárcano FM. Transl Oncol. 2024 Aug;46:102003. doi: 10.1016/j.tranon.2024.102003. Epub 2024 Jun 4. PMID: 38838438

Data documento
16/02/2023

Título
Impacto do uso excessivo da tecnologia na saúde dos jovens

Autor(es)
Natália Pavoni Catardo

Palavras chave
telas - celular

Descrição
Orientadora: Prof.ª Patrícia Modiano

Data documento
06/05/2024

Título
Impacto Psicossocial da Pandemia de COVID-19 em Trabalhadores de Instituição de Longa Permanência de Idosos

Autor(es)
MAIOLINO, Taís de Souza - REIS, Leticia Caldeira dos - COSTA, Ricardo Filipe Alves da - PETROUCIC, Roberta Thomé.

Palavras chave
Instituição de Longa Permanência para Idosos - Pessoal de saúde - Saúde mental - COVID-19

Resumo
Objetivos: Identificar o impacto psicossocial gerado pelo contexto pandêmico de COVID-19 nos cuidadores e profissionais da saúde de instituição de longa permanência de idosos e analisar as estratégias de enfretamento (coping) à pandemia. Métodos: Estudo observacional e transversal, aprovado por Comitê de Ética CAAE: 37255020.7.0000.5437. Realizado no período de outubro e novembro de 2020, com aplicação de questionários a cuidadores e profissionais da saúde da instituição “Casa Transitória André Luiz” de Barretos, estado de São Paulo. Foram aplicados os questionários: geral (sociodemográfico, laboral e testagem para COVID-19), o Self-Reporting Questionnaire (SRQ-20) para rastrear Distúrbios Psíquicos Menores (DPM) e o COPE-breve para elencar estratégias adotadas pelos trabalhadores no enfretamento à pandemia. Resultados: Foram incluídos no estudo 57 profissionais da saúde. Destes, 86,0% são do gênero feminino, 63,2% possuem escolaridade até o Ensino Médio/Técnico, 61,4% cumprem carga horária inferior a 40 horas semanais e 40,0% relataram já haver recebido testagem positiva para COVID-19. Considerado o escore maior ou igual a 7 no SRQ-20, foi rastreada a probabilidade de ocorrência de DPM em 35,1% dos trabalhadores, a qual não apresentou associação com as variáveis sociodemográficas, laborais e de testagem para COVID-19. As estratégias de enfrentamento mais utilizadas foram aquelas relacionadas à religião, aceitação, planejamento/coping ativo e reinterpretação positiva. Os profissionais de saúde com alta probabilidade de DPM apresentaram escores medianos mais elevados no suporte instrumental e na expressão de sentimentos, como estratégias de coping, do que os profissionais de saúde com baixa probabilidade de DPM. Conclusão: Foi identificada provável ocorrência de DPM entre profissionais da saúde da instituição. Esta se associou à busca por suporte instrumental e à expressão de sentimentos como estratégias de enfrentamento ao contexto de pandemia.

Citação
MAIOLINO, T. S.; REIS, L. C.; COSTA, R. F. A.; PETROUCIC, R. T.

Data documento
13/03/2025

Título
Implementation of the ProMisE classifier and validation of its prognostic impact in Brazilian endometrial carcinomas

Autor(es)
Andrade DAP, Bonatelli M, de Paula FE, Berardinelli GN, Teixeira GR, Dos Reis MT, Barbin FF, Andrade CEMDC, Aguiar VP, Hermoza AD, Hirai WY, Schmidt RL, Reis RM, Dos Reis R.

Palavras chave
Oncologia Ginecológica - Hospital do Amor Barretos SP

Resumo
Purpose Molecular classification of endometrial cancer (EC) has emerged as a key approach to individualize therapy and define prognostic outcomes. This study aimed to implement the traditional ProMisE classification in a Brazilian population, compared with a molecular setting of ProMisE biomarkers, and evaluate its impact on patients’ prognosis. Patient and methods A prospective cohort of 114 patients with primary EC treated at Barretos Cancer Hospital (BCH) between October 2020 and December 2022 was conducted. Pathology diagnosis, staging, treatment, and follow-up data were collected. The traditional ProMisE methodology was carried out by POLE hotspot sequencing and immunohistochemistry (IHC) for p53 and mismatch repair (MMR) proteins. We further evaluate the MMR and TP53 status by molecular approach, namely microsatellite instability (MSI) by PCR-based and TP53 mutation analysis by next-generation sequencing (NGS). The results of the 4 molecular groups in both methodologies were compared regarding agreement accuracy and survival outcomes. Results Among the 114 cases, the traditional ProMisE groups were: POLEmut 15.8%, MMRd 28.1%, p53abn 27.2%, and no specific molecular profile (NSMP) 28.9%. Considering the molecular classification approach, we observed a POLEmut group of 15.8%, MSI group of 23.7%, TP53 mutation of 27.2%, and NSMP of 33.3%. The concordance rate of both approaches was 86.8% (99/114 cases) with an overall accuracy of 0.87. Importantly, both traditional and molecular ProMisE approaches were associated with significant distinct overall survival (OS) and progression-free survival (PFS) outcomes, with POLEmut patients exhibiting a better prognosis (93.8% OS, at 24 months), whereas the p53abn having a worse survival time (68.9% of OS, at 24 months). Conclusion We reported for the first time the Brazilian profile of the ProMisE classification of endometrial cancer and demonstrated the prognostic impact of the traditional and molecular ProMisE classification on patient outcomes.

Data documento
14/01/2024

Título
Importância do exercício para saúde cardiovascular

Autor(es)
Mariana Olimpio dos Santos Remiro

Palavras chave
saúde - sedentarismo

Descrição
Orientador: Prof. Sérgio Luiz Brasileiro Lopes

Data documento
31/01/2024

Título
Imunoterapia: o futuro do tratamento contra o câncer

Autor(es)
Livia Lanzoni de Freitas

Palavras chave
T-CAR - imunoterapia

Descrição
Orientadora: Prof.ª Fabiana Albani Zambuzi Roberto

Data documento
05/10/2024

Título
Jovens também infartam!

Autor(es)
Bruno Mangiavacchi Silva

Palavras chave
coração - IAM - Infarto agudo do miocárdio

Descrição
Orientadora: Prof.ª Luciana Souza Jorge

Data documento
03/05/2024

Título
Liquid Biopsy for Lung Cancer: Up-to-Date and Perspectives for Screening Programs

Autor(es)
Casagrande GMS, - Silva MO, - Reis RM, - Leal LF.

Palavras chave
biomarkers - liquid biopsy - lung cancer - precision medicine

Resumo
Lung cancer is the deadliest cancer worldwide. Tissue biopsy is currently employed for the diagnosis and molecular stratification of lung cancer. Liquid biopsy is a minimally invasive approach to determine biomarkers from body fluids, such as blood, urine, sputum, and saliva. Tumor cells release cfDNA, ctDNA, exosomes, miRNAs, circRNAs, CTCs, and DNA methylated fragments, among others, which can be successfully used as biomarkers for diagnosis, prognosis, and prediction of treatment response. Predictive biomarkers are well-established for managing lung cancer, and liquid biopsy options have emerged in the last few years. Currently, detecting EGFR p.(Tyr790Met) mutation in plasma samples from lung cancer patients has been used for predicting response and monitoring tyrosine kinase inhibitors (TKi)-treated patients with lung cancer. In addition, many efforts continue to bring more sensitive technologies to improve the detection of clinically relevant biomarkers for lung cancer. Moreover, liquid biopsy can dramatically decrease the turnaround time for laboratory reports, accelerating the beginning of treatment and improving the overall survival of lung cancer patients. Herein, we summarized all available and emerging approaches of liquid biopsy-techniques, molecules, and sample type-for lung cancer.

Citação
Casagrande GMS, Silva MO, Reis RM, Leal LF. Liquid Biopsy for Lung Cancer: Up-to-Date and Perspectives for Screening Programs. Int J Mol Sci. 2023 Jan 28;24(3):2505. doi: 10.3390/ijms24032505. PMID: 36768828; PMCID: PMC9917347.

Data documento
06/05/2024

Título
Management of Early-Stage Vulvar Cancer

Autor(es)
Pedrão P.G. - Guimarães Y.M. - Godoy L.R. - Possati-Resende J.C. - Bovo A.C. - Andrade C.E.M.C. - Longatto-Filho A. - Dos Reis R.

Palavras chave
vulvar cancer - vulvar neoplasms - diagnosis and staging - sentinel lymph node

Resumo
Vulvar cancer is a rare gynecological malignancy since it represents 4% of all cancers of the female genital tract. The most common histological type is squamous cell carcinoma (90%). This type can be classified into two clinicopathological subtypes according to the etiology. The first subtype is associated with persistent human papillomavirus infection and is usually diagnosed in younger women. The second subtype is associated with lichen sclerosus condition, and in most cases is diagnosed in postmenopausal women. Currently, an increase in first subtype cases has been observed, which raised the concern about associated mortality and treatment morbidity among young women. Vulvar cancer treatment depends on histopathology grade and staging, but surgery with or without radiotherapy as adjuvant treatment is considered the gold standard. In recent decades, sentinel lymph node biopsy has been incorporated as part of the treatment. Therefore, we sought to review and discuss the advances documented in the literature about vulvar cancer focusing on the treatment of early-stage disease. Relevant articles, such as the GROINS-V studies and the GOG protocols, are presented in this review. Additionally, we discuss key points such as the evolution of treatment from invasive surgery with high morbidity, to more conservative approaches without compromising oncologic safety; the role of sentinel lymph node mapping in the initial staging, since it reduces the complications caused by inguinofemoral lymphadenectomy; the recurrences rates, since local recurrence is common and curable, however, groin-associated, or distant recurrences have a poor prognosis; and, finally, the long-term follow-up that is essential for all patients.

Citação
Pedrão PG, Guimarães YM, Godoy LR, Possati-Resende JC, Bovo AC, Andrade CEMC, Longatto-Filho A, Dos Reis R. Management of Early-Stage Vulvar Cancer. Cancers (Basel). 2022 Aug 29;14(17):4184. doi: 10.3390/cancers14174184. PMID: 36077719; PMCID: PMC9454625.

Data documento
03/05/2024

Título
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult

Autor(es)
da Silva FRM, - Zapaterini JR, - Grassi TF, - Bidinotto LT, - Fernandes AAH, - Barbisan LF.

Palavras chave
Development - mammary gland - rats - zinc - dimethylbenzanthracene

Resumo
We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.

Citação
da Silva FRM, Zapaterini JR, Grassi TF, Bidinotto LT, Fernandes AAH, Barbisan LF. Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult. Biotech Histochem. 2023 Nov;98(5):360-371. doi: 10.1080/10520295.2023.2196092. Epub 2023 Apr 6. PMID: 37022146.

Data documento
06/05/2024

Título
Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood

Autor(es)
Zapaterini J.R. - Fonseca A.R.B. - Bidinotto L.T. - Colombelli K.T. - Rossi A.L.D. - Kass L. - Justulin L.A. - Barbisan L.F.

Palavras chave
DNA damage - DNA repair and replication - N-methyl-N-nitrosourea - female Sprague–Dawley - maternal low protein intake - perinatal programming - risk for mammary carcinogenesis

Resumo
Studies have shown that maternal malnutrition, especially a low-protein diet (LPD), plays a key role in the developmental mechanisms underlying mammary cancer programming in female offspring. However, the molecular pathways associated with this higher susceptibility are still poorly understood. Thus, this study investigated the adverse effects of gestational and lactational low protein intake on gene expression of key pathways involved in mammary tumor initiation after a single dose of N-methyl-N-nitrosourea (MNU) in female offspring rats. Pregnant Sprague-Dawley rats were fed a normal-protein diet (NPD) (17% protein) or LPD (6% protein) from gestational day 1 to postnatal day (PND) 21. After weaning (PND 21), female offspring (n = 5, each diet) were euthanized for histological analysis or received NPD (n = 56 each diet). At PND 28 or 35, female offspring received a single dose of MNU (25 mg/kg body weight) (n = 28 each diet/timepoint). After 24 h, some females (n = 10 each diet/timepoint) were euthanized for histological, immunohistochemical, and molecular analyses at PDN 29 or 36. The remaining animals (n = 18 each diet/timepoint) were euthanized when tumors reached ≥2 cm or at PND 250. Besides the mammary gland development delay observed in LPD 21 and 28 groups, the gene expression profile demonstrated that maternal LPD deregulated 21 genes related to DNA repair and DNA replication pathways in the mammary gland of LPD 35 group after MNU. We further confirmed an increased γ-H2AX (DNA damage biomarker) and in ER-α immunoreactivity in mammary epithelial cells in the LPD group at PND 36. Furthermore, these early postnatal events were followed by significantly higher mammary carcinogenesis susceptibility in offspring at adulthood. Thus, the results indicate that maternal LPD influenced the programming of chemically induced mammary carcinogenesis in female offspring through increase in DNA damage and deregulation of DNA repair and DNA replication pathways. Also, Cidea upregulation gene in the LPD 35 group may suggest that maternal LPD could deregulate genes possibly leading to increased risk of mammary cancer development and/or poor prognosis. These findings increase the body of evidence of early-transcriptional mammary gland changes influenced by maternal LPD, resulting in differential response to breast tumor initiation and susceptibility and may raise discussions about lifelong prevention of breast cancer risk.

Citação
Zapaterini JR, Fonseca ARB, Bidinotto LT, Colombelli KT, Rossi ALD, Kass L, Justulin LA, Barbisan LF. Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood. Front Cell Dev Biol. 2022 Feb 1;9:756616. doi: 10.3389/fcell.2021.756616. PMID: 35178394; PMCID: PMC8844450.

Data documento
02/05/2024

Título
Metodologias ativas de aprendizagem na capacitação de enfermeiros para a prevenção de lesão por pressão

Autor(es)
Assoni, M. A. S. - Oliveira, É. M. M. - Cardoso, D. M. - Lourenço, A. O. O. S. - Alvarenga, C. S.

Palavras chave
autocuidado - teoria da enfermaria - bibliometria - teses como temas

Resumo
Caracterizar a produção científica relacionada à Teoria do Autocuidado de OREM nas teses de enfermagem brasileiras disponíveis no banco de testes do portal CAPES. Método: Revisão bibliométrica de caráter descritivo, desenvolvida a partir da análise de teses disponíveis na CAPES. Pesquisa descritiva com Análise de Conteúdo, desenvolvida pela categorização dos conteúdos das teses conforme a utilização da teoria do autocuidado. Resultados: Foram incluídas 10 teses que apresentaram diferentes formas de aplicabilidade da teoria de Orem validação de protocolo e escala, adaptação, desenvolvimento da consulta de enfermagem e elaboração de instrumento norteador da consulta de enfermagem. As estruturas semânticas relacionadas às categorizações principais de utilização da teoria do autocuidado envolveram ação, instrumento, condições básicas e suplementares do ser humano e lócus do cuidado. Conclusão: A Teoria de Orem foi utilizada em contextos diversificados, fomentando caminhos para reflexões sobre a construção e aplicação do conhecimento próprio da enfermagem.

Citação
Assoni, M. A. S.; Oliveira, É. M. M.; Cardoso, D. M.; Lourenço, A. O. O. S.; Alvarenga, C. S. Metodologias ativas de aprendizagem na capacitação de enfermeiros para a prevenção de lesão por pressão, Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-1372463

Data documento
01/12/2020

Título
Mobilização precoce em pacientes no pós-operatório de cirurgia cardíaca: revisão integrativa

Autor(es)
Ana Paula Marques Natividade - Laira Joice Tashima

Palavras chave
Cirurgia Cardíaca - Mobilização precoce - Fisioterapia

Resumo
INTRODUÇÃO: No pós-operatório de cirurgia cardíaca, devido ao repouso prolongado, podem ocorrer diversas complicações. Com os avanços científicos e tecnológicos compreendemos a importância da adoção da mobilização precoce pelos fisioterapeutas na Unidade de Terapia Intensiva (UTI). Dessa forma aumentaremos a força muscular e a função física do paciente com exercícios de mobilidade no leito e a deambulação OBJETIVOS: Evidenciar a eficácia da mobilização precoce na recuperação de paciente em pós-operatório de cirurgia cardíaca. MATERIAIS E MÉTODOS: Trata-se de uma revisão integrativa e para isso foram utilizadas as bases de dados: Scielo, LILACS, PUBMED, Biblioteca Virtual em Saúde (BVS)e Google Acadêmico; em português e espanhol, publicados nos últimos 10 anos. RESULTADOS: Foram selecionados 12 artigos que atingem os objetivos de estudo deste Trabalho. CONCLUSÃO: Com a realização da mobilidade precoce em pacientes do pós-operatório de cirurgia cardíaca, observamos que houve uma melhora na força muscular respiratória e periférica, diminuição do tempo de internação e uso da ventilação mecânica.

Descrição
(Pós Graduação em Fisioterapia Urgência/Emergência e Terapia Intensiva)

Citação
NATIVIDADE, Ana Paula Marques; TASHIMA, Laira Joice. Mobilização precoce em pacientes no pós-operatório de cirurgia cardíaca: revisão integrativa. 2020. 20f. Trabalho de Conclusão de Curso (Pós Graduação em Fisioterapia Urgência/Emergência e Terapia Intensiva) - Faculdade de Ciências da Saúde de Barretos Dr Paulo Prata, Barretos, 2020.

Data documento
13/03/2025

Título
Molecular profile of driver genes in lung adenocarcinomas of Brazilian patients who have never smoked: implications for targeted therapies

Autor(es)
Rodrigo de Oliveira Cavagna - Flávia Escremim de Paula - Gustavo Noriz Berardinelli - Murilo Bonatelli - Iara Santana - Eduardo Caetano Albino da Silva - Gustavo Ramos Teixeira - Beatriz Garbe Zaniolo - Flávio Augusto Ferreira da Silva - Carlos Eduardo Baston Silva - Josiane Mourão Dias - Marcela Gondim Borges Guimarães - Camila Pinto Barone - Alexandre Arthur Jacinto - Rachid Eduardo Noleto da Nóbrega Oliveira - José Elias Miziara - Pedro De Marchi - Miguel A. Molina-Vila - Letícia Ferro Leal - Rui Manuel Reis

Palavras chave
lung adenocarcinoma; molecular profile; driver mutations; never smoker; Latin America.

Resumo
Introduction: Lung cancer in never-smoker (LCINS) patients accounts for 20% of lung cancer cases, and its biology remains poorly understood, particularly in genetically admixed populations. We elucidated the molecular profile of driver genes in Brazilian LCINS. Methods: The mutational and gene fusion status of 119 lung adenocarcinomas from self-reported never-smoker patients, was assessed using targeted sequencing (NGS), nCounter, and immunohistochemistry. A panel of 46 ancestry-informative markers determined patients’ genetic ancestry. Results: The most frequently mutated gene was EGFR (49.6%), followed by TP53 (39.5%), ALK (12.6%), ERBB2 (7.6%), KRAS (5.9%), PIK3CA (1.7%), and less than 1% alterations in RET, NTRK1, MET∆ex14, PDGFRA, and BRAF. Except for TP53 and PIK3CA, all other alterations were mutually exclusive. Genetic ancestry analysis revealed a predominance of European (71.1%), and a higher African ancestry was associated with TP53 mutations. Conclusion: Brazilian LCINS exhibited a similar molecular profile to other populations, except the increased ALK and TP53 alterations. Importantly, 73% of these patients have actionable alterations that are suitable for targeted treatments.

Citação
Cavagna RO, Escremim de Paula F, Berardinelli GN, Bonatelli M, Santana I, Albino da Silva EC, Teixeira GR, Zaniolo BG, Mourão Dias J, Ferreira da Silva FA, Baston Silva CE, Guimarães MGB, Barone CP, Jacinto AA, Noleto da Nóbrega Oliveira RE, Miziara JE, De Marchi P, Molina-Vila MA, Leal LF, Reis RM. Oncologist. 2024 Oct 3;29(10):e1419-e1424. doi: 10.1093/oncolo/oyae129. PMID: 38944844

Data documento
13/11/2024

Título
Muita tela, pouco movimento: o sono dos jovens em ameaça

Autor(es)
Marcela Viscovini Gomes da Silva

Palavras chave
adolescente - celular

Descrição
Orientadora: Prof.ª Barbara Sgavioli Massucato

Data documento
31/07/2025

Título
O direito de planejar

Autor(es)
Helena Marques e Silva

Palavras chave
Gravidez - planejamento familiar

Descrição
Orientadora: Prof.ª Vânia Soares de Oliveira e Almeida Pinto

Data documento
26/05/2023

Título
O uso de telas deve ser monitorado pelos pais?

Autor(es)
Lívia Siqueira da Silva

Palavras chave
celular - tecnologia

Descrição
Orientadora: Prof.ª Rosalina Massako Yamawaki Murata

Data documento
07/05/2025

Título
O Zé Gotinha não existe mais?

Autor(es)
Giovanna Christina Treviso Garofo

Palavras chave
vacina - Poliomielite - zé gotinha - paralisia infantil

Descrição
Orientadora: Prof.ª Daniele Natália Pacharone Bertolini Bidonotto

Data documento
14/06/2024

Título
Os impactos do álcool e do tabaco na saúde fetal

Autor(es)
Maria Laura Bomfim Nascimento

Palavras chave
cigarro - agentes teratogênicos

Descrição
Orientador: Prof. Lucas Tadeu Bidinotto

Data documento
30/06/2023

Título
Os passos para parar de fumar

Autor(es)
Victoria Borges Bessa

Palavras chave
cigarro - tabagismo

Descrição
Orientadora: Prof.ª Gabriela Rossi Zaia

Data documento
28/10/2023

Título
Os riscos de ser um fumante passivo

Autor(es)
Lívia Bertolo Gonzaga

Palavras chave
cigarro - tabaco

Descrição
Orientadora: Prof.ª Ludmila Pereira Barbosa dos Santos

Data documento
06/05/2024

Título
Paracoccidioidomycosis with sarcoid-like cutaneous lesion: A clinicopathological challenge

Autor(es)
Cárcano C.B.M. - Tanaka V.D. - Alessi C. - Reis M.T. - Ferreira M.S.

Palavras chave
Paracoccidioidomycosis - Mycoses - Histology - Dermatology - Diagnosis - Sarcoidosis

Resumo
We describe a case of sarcoid-like cutaneous paracoccidioidomycosis in a 26-year-old male, with a 10-year evolution, wrongly diagnosed as granulomatous rosacea. The correct diagnosis was only possible after the appearance of a new skin lesion with a more typical characteristic of the dermatosis, correlated with anatomopathological, laboratory and imaging exams. The clinical presentation of paracoccidioidomycosis is diverse, and the sarcoid-like form can mimic several chronic granulomatous diseases, such as sarcoidosis, tuberculoid leprosy, leishmaniasis, or tuberculosis. This presentation of cutaneous paracoccidioidomycosis is rare, and its diagnosis depends on the clinicopathological correlation, which can be a challenge for the dermatologist.

Citação
Cárcano CBM, Tanaka VD, Alessi C, Reis MT, Ferreira MS. Paracoccidioidomycosis with sarcoid-like cutaneous lesion: A clinicopathological challenge. IDCases. 2022 Jul 18;29:e01574. doi: 10.1016/j.idcr.2022.e01574. PMID: 35923859; PMCID: PMC9340507.

Data documento
26/04/2024

Título
PD L1 expression and microsatellite instability (MSI) in cancer of unknown primary site

Autor(es)
JÚNIOR, João Neif Antonio - PRETO, Daniel D.’Almeida - LAZARINI, Maria Eduarda Zanatta Neder - LIMA, Marcos Alves de - BONATELLI, Murilo - BERARDINELLI, Gustavo Noriz - SILVA, Vinicius Duval da - PINHEIRO, Céline - REIS, Rui Manuel, CÁRCANO, Flavio Mavignier -

Palavras chave
Neoplasms - Unknown primary - Microsatellite instability - Immune checkpoint proteins - Immunotherapy

Resumo
Background Cancer of unknown primary site (CUP) is a heterogeneous group of tumors for which the origin remains unknown. Clinical outcomes might be influenced by regulatory processes in its microenvironment. Microsatellite instability (MSI) is a predictive biomarker for cancer immunotherapy and its status, as well as co-occurrence with PD-L1 expression, is poorly evaluated. We aim to evaluate the expression of PD-L1 and the status of MSI in CUP and their possible associations with clinical–pathological features. Methods The combined positive score (CPS) PD-L1 expression was evaluated by immunohistochemistry. MSI status was assessed using a hexa-plex marker panel by polymerase chain reaction followed by fragment analysis. Results Among the 166 cases, MSI analysis was conclusive in 120, with two cases being MSI positive (1.6%). PD-L1 expression was positive in 18.3% of 109 feasible cases. PD-L1 expression was significantly associated with non-visceral metastasis and a dominance of nodal metastasis. The median overall survival (mOS) was 3.7 (95% CI 1.6–5.8) months and patients who expressed PD-L1 achieved a better mOS compared to those who did not express PD-L1 (18.7 versus 3.0 months, p-value: < .001). ECOG-PS equal to or more than two and PD-L1 expression were independent prognostic factors in multivariate analysis (2.37 and 0.42, respectively). Conclusion PD-L1 is expressed in a subset (1/5) of patients with CUP and associated with improved overall survival, while MSI is a rare event. There is a need to explore better the tumor microenvironment as well as the role of immunotherapy to change such a bad clinical outcome.

Citação
Junior JNA, Preto DD, Lazarini MEZN, de Lima MA, Bonatelli M, Berardinelli GN, da Silva VD, Pinheiro C, Reis RM, Cárcano FM. PD-L1 expression and microsatellite instability (MSI) in cancer of unknown primary site. Int J Clin Oncol. 2024 Mar 25. doi: 10.1007/s10147-024-02494-3. Epub ahead of print. PMID: 38528294.

Data documento
19/03/2025

Título
PD-L1 expression and microsatellite instability (MSI) in cancer of unknown primary site

Autor(es)
João Neif Antonio Junior - Daniel D Almeida Preto - Maria Eduarda Zanatta Neder Lazarini - Marcos Alves de Lima - Murilo Bonatelli - Gustavo Noriz Berardinelli - Vinicius Duval da Silva - Céline Pinheiro - Rui Manuel Reis - Flavio Mavignier Cárcano.

Palavras chave
Immune checkpoint proteins; Immunotherapy; Microsatellite instability; Neoplasms; Unknown primary.

Resumo
Background: Cancer of unknown primary site (CUP) is a heterogeneous group of tumors for which the origin remains unknown. Clinical outcomes might be influenced by regulatory processes in its microenvironment. Microsatellite instability (MSI) is a predictive biomarker for cancer immunotherapy and its status, as well as co-occurrence with PD-L1 expression, is poorly evaluated. We aim to evaluate the expression of PD-L1 and the status of MSI in CUP and their possible associations with clinical-pathological features. Methods: The combined positive score (CPS) PD-L1 expression was evaluated by immunohistochemistry. MSI status was assessed using a hexa-plex marker panel by polymerase chain reaction followed by fragment analysis. Results: Among the 166 cases, MSI analysis was conclusive in 120, with two cases being MSI positive (1.6%). PD-L1 expression was positive in 18.3% of 109 feasible cases. PD-L1 expression was significantly associated with non-visceral metastasis and a dominance of nodal metastasis. The median overall survival (mOS) was 3.7 (95% CI 1.6-5.8) months and patients who expressed PD-L1 achieved a better mOS compared to those who did not express PD-L1 (18.7 versus 3.0 months, p-value: < .001). ECOG-PS equal to or more than two and PD-L1 expression were independent prognostic factors in multivariate analysis (2.37 and 0.42, respectively). Conclusion: PD-L1 is expressed in a subset (1/5) of patients with CUP and associated with improved overall survival, while MSI is a rare event. There is a need to explore better the tumor microenvironment as well as the role of immunotherapy to change such a bad clinical outcome.

Citação
unior JNA, Preto DD, Lazarini MEZN, de Lima MA, Bonatelli M, Berardinelli GN, da Silva VD, Pinheiro C, Reis RM, Cárcano FM.Int J Clin Oncol. 2024 Jun;29(6):726-734. doi: 10.1007/s10147-024-02494-3.Epub 2024 Mar 25. PMID: 38528294

Data documento
17/04/2023

Título
Pobreza Menstrual no Brasil : causas políticas e culturais

Autor(es)
Ana Beatriz Franco Santos

Palavras chave
carência - carente - população

Descrição
Orientadora: Prof.ª Patrícia Modiano

Data documento
05/05/2019

Título
Prós e contras do atendimento hospitalar versus atendimento domiciliar em cuidados paliativos

Autor(es)
Ana Lucia do Nascimento Braga

Palavras chave
Oncologia - Assistência a Saúde - Cuidados Paliativos - Atendimento Domiciliar

Resumo
JUSTIFICATIVA: trabalhando em um Hospital Oncológico ao se deparar com o momento em que a família é informada que o paciente apenas poderá receber cuidados paliativos e observar o quão triste, isto é, no contexto familiar. O lidar com a revolta e a negação da situação, e a enfermagem que cuida do paciente sem expectativa de cura. E ao vivenciar isso o quanto e difícil para envolver toda equipe. OBJETIVO: observar e avaliar os prós e contras da atenção domiciliar e hospitalar. MATERIAIS E METODOS: busca de artigos na literatura utilizando banco de dados. RESULTADOS: Após a busca de artigos por conveniência, finalizamos o trabalho com treze (13) artigos. CONCLUSÃO: concluiu-se que apesar de ser destacado prós e contras específicos, ainda há muitas variáveis para optar por um ou outro método.

Descrição
Trabalho de Conclusão de Curso (Pós Graduação em Oncologia)

Citação
BRAGA, Ana Lúcia Nascimento. Prós e contras do atendimento hospitalar vs atendimento domiciliar em cuidados paliativos. 2019. 10f. Trabalho de Conclusão de Curso (Pós Graduação em Oncologia) - Faculdade de Ciências da Saúde de Barretos Dr. Paulo Prata. Barretos, 2019.

Data documento
04/01/2023

Título
Quais e quantos tipos de dores de cabeça existem?

Autor(es)
Robson Aparecido dos Santos Boni

Palavras chave
cefaleia - aneurisma arterial - hemorragia intracraniana

Descrição
Biomédico e docente do curso de Medicina da FACISB

Data documento
12/02/2023

Título
Rastreamento de câncer de pulmão em pacientes fumantes ou ex-fumantes

Autor(es)
Ludmila Pereira Barbosa dos Santos

Palavras chave
cigarro - tabagismo

Descrição
Médica Pneumologista e docente do curso de Medicina da FACISB

Data documento
26/04/2025

Título
Sarampo: uma doença grave, mas prevenível!

Autor(es)
Brenda Rafaela Romão Queiroz

Palavras chave
sarampo - doença viral

Descrição
Orientadora: Prof.ª. Luciana Souza Jorge

Data documento
02/05/2024

Título
Sarcopenia in Children With Wilms Tumor: A Marker of Undernutrition Which May Impact Adversely on Clinical Outcomes

Autor(es)
de Oliveira WE Jr, - Murra MS, - Tufi LMB, - Cavalcante CEB, - de Oliveira MA, - da Costa RFA, - Rosa BR, - da Silva RZM, - Ribeiro RC, - Ladas EJ, - Barr RD.

Palavras chave
sarcopenia - psoas muscle area - Wilms tumor

Resumo
The therapeutic approach to Wilms tumor (WT) is multidisciplinary and leads to significant patient impairment, increasing the risk of nutritional compromise and malnutrition. Children with cancer are vulnerable to sarcopenia which has been recognized as a negative impact of anticancer therapy. Recent studies have highlighted the reduction in the total psoas muscle area (TPMA) to be associated with a poor prognosis inmany pediatric diseases, including cancer. This study aims to evaluate changes in the TPMA compartment during the treatment of children with WT. An observational, longitudinal, and retrospective study was undertaken in a single institution evaluating children (1 to 14 y, n=38) with WT between 2014 and 2020. TPMA was assessed by the analysis of previously collected, electronically stored computed tomography images of the abdomen obtained at 3 time points: diagnosis, preoperatively, and 1 year after surgery. For all patients, TPMA/age were calculated with a specific online calculator. Our data show a high incidence of sarcopenia (55.3%) at diagnosis which increased after 4 to 6 weeks of neoadjuvant chemotherapy (73.7%) and remained high (78.9%) 1 year after the surgical procedure. Using TPMA/age Z-score curves we have found significant and rapid muscle loss in children with WT, with little or no recovery in the study period.

Citação
de Oliveira WE Jr, Murra MS, Tufi LMB, Cavalcante CEB, de Oliveira MA, da Costa RFA, Rosa BR, da Silva RZM, Ribeiro RC, Ladas EJ, Barr RD. Sarcopenia in Children With Wilms Tumor: A Marker of Undernutrition Which May Impact Adversely on Clinical Outcomes. J Pediatr Hematol Oncol. 2023 Oct 1;45(7):e817-e822. doi: 10.1097/MPH.0000000000002732. Epub 2023 Jul 31. PMID: 37526408.

Data documento
04/01/2023

Título
Saúde mental dos profissionais da saúde de Instituições de Longa Permanência

Autor(es)
Taís de Souza Maiolino

Palavras chave
ILP - bem-estar

Descrição
Orientador: Prof. João Luiz Brisotti e Prof.ª Barbara Sgavioli Massucato

Data documento
04/12/2024

Título
Saúde Mental e os impactos da pandemia

Autor(es)
Maria Eduarda Franco de Paula

Palavras chave
COVID-19 - bem-estar - isolamento

Descrição
Orientador: Prof. Eduardo Marcelo Cândido

Data documento
18/12/2023

Título
SERS sensing for cancer biomarker: Approaches and directions

Autor(es)
Lorena Vázquez-Iglesias - Giovanna Maria Stanfoca Casagrande - Daniel García-Lojo - Letícia Ferro Leal - Tien Anh Ngo - Jorge Pérez-Juste - Rui Manue

Palavras chave
Cancer biomarkers; Invasive and non-invasive biomarkers; Microfluidic devices; Point of care detection; Surface-enhanced Raman scattering (SERS).

Resumo
These days, cancer is thought to be more than just one illness, with several complex subtypes that require different screening approaches. These subtypes can be distinguished by the distinct markings left by metabolites, proteins, miRNA, and DNA. Personalized illness management may be possible if cancer is categorized according to its biomarkers. In order to stop cancer from spreading and posing a significant risk to patient survival, early detection and prompt treatment are essential. Traditional cancer screening techniques are tedious, time-consuming, and require expert personnel for analysis. This has led scientists to reevaluate screening methodologies and make use of emerging technologies to achieve better results. Using time and money saving techniques, these methodologies integrate the procedures from sample preparation to detection in small devices with high accuracy and sensitivity. With its proven potential for biomedical use, surface-enhanced Raman scattering (SERS) has been widely used in biosensing applications, particularly in biomarker identification. Consideration was given especially to the potential of SERS as a portable clinical diagnostic tool. The approaches to SERS-based sensing technologies for both invasive and non-invasive samples are reviewed in this article, along with sample preparation techniques and obstacles. Aside from these significant constraints in the detection approach and techniques, the review also takes into account the complexity of biological fluids, the availability of biomarkers, and their sensitivity and selectivity, which are generally lowered. Massive ways to maintain sensing capabilities in clinical samples are being developed recently to get over this restriction. SERS is known to be a reliable diagnostic method for treatment judgments. Nonetheless, there is still room for advancement in terms of portability, creation of diagnostic apps, and interdisciplinary AI-based applications. Therefore, we will outline the current state of technological maturity for SERS-based cancer biomarker detection in this article. The review will meet the demand for reviewing various sample types (invasive and non-invasive) of cancer biomarkers and their detection using SERS. It will also shed light on the growing body of research on portable methods for clinical application and quick cancer detection.

Citação
SERS sensing for cancer biomarker: Approaches and directions. Vázquez-Iglesias L, Stanfoca Casagrande GM, García-Lojo D, Ferro Leal L, Ngo TA, Pérez-Juste J, Reis RM, Kant K, Pastoriza-Santos I. Bioact Mater. 2023 Dec 31;34:248-268. doi: 10.1016/j.bioactmat.2023.12.018. eCollection 2024 Apr. PMID: 38260819

Data documento
03/05/2024

Título
Somatic mutation detection and KRAS amplification in testicular germ cell tumors

Autor(es)
Cabral ERM, - Pacanhella MF, - Lengert AVH, - Dos Reis MB, - Leal LF, - de Lima MA, - da Silva ALV, - Pinto IA, - Reis RM, - Pinto MT, - Cárcano FM.

Palavras chave
K-ras Gene - TP53 Gene - mutation - next-generation sequencing - testicular germ cell tumor - testicular neoplasms

Resumo
Background: Testicular Germ Cell Tumors (TGCT) are the most common cancer among young adult men. The TGCT histopathology is diverse, and the frequency of genomic alterations, along with their prognostic role, remains largely unexplored. Herein, we evaluate the mutation profile of a 15-driver gene panel and copy number variation of KRAS in a large series of TGCT from a single reference cancer center. Materials and methods: A cohort of 97 patients with TGCT, diagnosed at the Barretos Cancer Hospital, was evaluated. Real-time PCR was used to assess copy number variation (CNV) of the KRAS gene in 51 cases, and the mutation analysis was performed using the TruSight Tumor 15 (Illumina) panel (TST15) in 65 patients. Univariate analysis was used to compare sample categories in relation to mutational frequencies. Survival analysis was conducted by the Kaplan-Meier method and log-rank test. Results: KRAS copy number gain was a very frequent event (80.4%) in TGCT and presented a worse prognosis compared with the group with no KRAS copy gain (10y-OS, 90% vs. 81.5%, p = 0.048). Among the 65 TGCT cases, different variants were identified in 11 of 15 genes of the panel, and the TP53 gene was the most recurrently mutated driver gene (27.7%). Variants were also detected in genes such as KIT, KRAS, PDGFRA, EGFR, BRAF, RET, NRAS, PIK3CA, MET, and ERBB2, with some of them potentially targetable. Conclusion: Although larger studies incorporating collaborative networks may shed the light on the molecular landscape of TGCT, our findings unveal the potential of actionable variants in clinical management for applying targeted therapies.

Citação
Cabral ERM, Pacanhella MF, Lengert AVH, Dos Reis MB, Leal LF, de Lima MA, da Silva ALV, Pinto IA, Reis RM, Pinto MT, Cárcano FM. Somatic mutation detection and KRAS amplification in testicular germ cell tumors. Front Oncol. 2023 Mar 16;13:1133363. doi: 10.3389/fonc.2023.1133363. PMID: 37007070; PMCID: PMC10060882.

Data documento
10/05/2023

Título
Tabagismo e câncer de pulmão no Brasil

Autor(es)
Gabriela Camargo Guindalini

Palavras chave
tabaco - cigarro

Descrição
Orientadora: Prof.ª Leticia Leal

Data documento
02/05/2024

Título
The digital expression profile of BMP7, CDKN2C, HIST1H3G, and PKMYT1 genes improves high‐grade cervical lesion detection in liquid‐based cytology

Autor(es)
Causin RL, - Sussuchi da Silva L, - Leal LF, - Possati-Resende JC, - Evangelista AF, - Matsushita GM, - Scapulatempo-Neto C, - Tavares Guerreiro Fregnani JH, - Antônio de Oliveira M, - Musselwhite LW, - Chiquitelli Marques MM, - Reis RM.

Palavras chave
biomarkers - cervical cancer - cervical intraepithelial neoplasia - gene expression - liquid‐based cytology

Resumo
Background: Some studies reported that differential gene expression could be used as a biomarker for high‐grade cervical lesion identification. The aim was to evaluate the gene expression profile of cervical intraepithelial neoplasia (CIN) to identify a gene expression signature of CIN2+ in liquid‐based cytology (LBC) samples. Methods: LBC samples (n = 85) obtained from women who underwent colposcopy were included with benign (n = 13), CIN1 (n = 26), CIN2 (n = 16), and CIN3 (n = 30) diagnoses. After RNA isolation, gene expression profiling was performed using the nCounter PanCancer Pathways, which consists of 730 cancer‐related genes. The genes identified were in silico expression evaluated using the UALCAN database. An accurate prediction model to discriminate CIN2+ from

Descrição

Citação
Causin RL, Sussuchi da Silva L, Leal LF, Possati-Resende JC, Evangelista AF, Matsushita GM, Scapulatempo-Neto C, Tavares Guerreiro Fregnani JH, Antônio de Oliveira M, Musselwhite LW, Chiquitelli Marques MM, Reis RM. The digital expression profile of BMP7, CDKN2C, HIST1H3G, and PKMYT1 genes improves high-grade cervical lesion detection in liquid-based cytology. Cancer Cytopathol. 2023 Jul;131(7):454-464. doi: 10.1002/cncy.22704. Epub 2023 Apr 17. PMID: 37069588.

Data documento
28/06/2025

Título
Tosse com pigarro, cansaço e noites mal dormidas: podem não ser só da idade!

Autor(es)
Luiza Calderelli Raposo

Palavras chave
tosse - pigarro

Descrição
Orientadora: Prof.ª Ludmila Pereira Barbosa dos Santos Carvalho

Data documento
25/07/2024

Título
Transplante de córnea e sua contribuição ao retorno da visão

Autor(es)
Maria Victória Marconi Cardoso Lombardi

Palavras chave
deficiência visual - tratamento ocular

Descrição
Orientador: Prof. Rodrigo Chaves Ribeiro